![]() ![]() We will go over possible answers to the question next meeting Think about the questions, but don’t answer them yet. There will be two questions asking why you think it’s unreadable. ![]() If you think it’s unreadable (I sort of gave it away), then when you click “next”, then it takes you to the analysis page. ![]() If your sequence has any of those, then it’s most likely unreadable. Does the middle of the sequence look like that? Are the wavelengths just plain sloppy? If you look to the beginning and to the very end, you’ll see that the peaks are “sloppy” and uneven. Are there clear, evenly spaced peaks in the sequence? Does the machine have many “N” nucleotides (ones that it can’t figure out what it is)? Is there “noise” beneath the peaks of the sequence? Though the machine may say that the sequence is what it is, check if there are other peaks beneath the sequence that shouldn’t be there. When we say readable what we mean is: Check what the machine reads. When you have clicked that button, it should take you to a page that looks like this. When you get there, click the green button “Next”. Click on “My Clones” and then click on “PC1.11” Log into you DSAP homepage like last time. You should also know about how the DNA is sequenced and what to look for when first analyzing the gene (the machine records the nucleotide in a sequence, but is prone to error).Īt this point, we are going to get into the actual DNA analysis on DSAP. Ok, by now, you should have downloaded PC1.11 and have the software to analyze it (4peaks for macs, Finch TV for windows) ![]()
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